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Image Search Results
Journal: Cancer Gene Therapy
Article Title: RNA interference-mediated knockdown of p21 WAF1 enhances anti-tumor cell activity of oncolytic adenoviruses
doi: 10.1038/cgt.2009.29
Figure Lengend Snippet: Viability of HCT116 cell lines transfected with p21 WAF or p27 KIP1 siRNAs before adenovirus infection. HCT116-WT or HCT116 p53−/− were transfected with p21 WAF1 or p27 KIP1 siRNAs. Adenovirus infections were carried out 48 h later. For HCT116 cells, viability was assessed 72 h after addition of the wild-type adenovirus (WtD) or Delta-24 adenoviruses or 94 h for ONYX-015. For HCT116 p53−/− cells, viability was assessed 72 h after addition of the WtD or 94 h for ONYX-015 and Delta-24. Western blot analysis of p21 WAF1 ( a and c ) or p27 KIP1 ( b and d ) expression in HCT116-WT cells transfected with p21 WAF1 (oligos #11 and #12), p27 KIP1 (oligos #5 and #8), or non-targeting control siRNAs. β-actin was used as loading control. Cell viability of p21 WAF1 or p27 KIP1 siRNA-transfected HCT116-WT ( e – g ) or HCT116 p53−/− ( h – j ) cells followed by WtD ( e and h ), ONYX-015 ( f and i ) or Delta-24 ( g and j ) infection. Viability is expressed as a relative percentage of cells transfected with non-targeting siRNA control. Error bars indicate s.d. * P <0.05, ** P <0.005 compared with negative control.
Article Snippet: Only a reduction of the viability to 60% after Delta-24 was observed after transfection of
Techniques: Transfection, Infection, Western Blot, Expressing, Control, Negative Control
Journal: Cancer Gene Therapy
Article Title: RNA interference-mediated knockdown of p21 WAF1 enhances anti-tumor cell activity of oncolytic adenoviruses
doi: 10.1038/cgt.2009.29
Figure Lengend Snippet: Replication efficiency of adenovirus in HCT116-WT or HCT116 p53−/− cells transfected with p21 WAF1 or p27 KIP1 siRNAs. HCT116-WT ( a – c ) or HCT116 p53−/− ( d – f ) cells were transfected with p21 WAF1 or p27 KIP1 siRNAs for 48 h. The viral titers were determined 72 h after infection with wild-type adenovirus (WtD) ( a and d ) or ONYX-015 ( b and e ) or Delta-24 ( c and f ). Results are shown as means of duplicate experiments. Error bars represent s.d. * P <0.05, ** P <0.005 compared with negative control.
Article Snippet: Only a reduction of the viability to 60% after Delta-24 was observed after transfection of
Techniques: Transfection, Infection, Negative Control
Journal: Cancer Gene Therapy
Article Title: RNA interference-mediated knockdown of p21 WAF1 enhances anti-tumor cell activity of oncolytic adenoviruses
doi: 10.1038/cgt.2009.29
Figure Lengend Snippet: Viability of TE7 ( b ), H1299 ( c ), DU145 ( d ) and MIA PaCa-2 ( e ) cells transfected with p21 WAF1 siRNAs before adenovirus infections. Cells were transfected with p21 WAF1 siRNA for 48 h. Western blotting of p21 WAF1 ( a ) in cells transfected with p21 WAF1 siRNA or non-targeting siRNA. β-actin was used as loading control. Cell viability was measured at 72 h after infection with wild-type adenovirus (WtD), ONYX-015 or Delta-24. Data is represented as cell viability relative to untreated cells (equal to 100%). * P <0.05, ** P <0.005 compared with negative control.
Article Snippet: Only a reduction of the viability to 60% after Delta-24 was observed after transfection of
Techniques: Transfection, Western Blot, Control, Infection, Negative Control
Journal: Cancer Gene Therapy
Article Title: RNA interference-mediated knockdown of p21 WAF1 enhances anti-tumor cell activity of oncolytic adenoviruses
doi: 10.1038/cgt.2009.29
Figure Lengend Snippet: Replication efficiency of adenovirus in TE7, H1299, DU145 and MIA PaCa-2 cells transfected with p21 WAF1 siRNAs for 48 h. The viral titers were determined at 72 h after infection with wild-type adenovirus (WtD), ONYX-015 or Delta-24. Results are shown as means of duplicate experiments. Data are represented as relative percentage of cells transfected with non-targeting siRNA control (equal to 100%). Error bars represent s.d. ** P <0.005 compared with negative control.
Article Snippet: Only a reduction of the viability to 60% after Delta-24 was observed after transfection of
Techniques: Transfection, Infection, Control, Negative Control